Part:BBa_K3697012
Signal Amplifier by B. subtilis Quorum Sensing Molecule comX
This is a signal amplification device that allows a signal from a few cells to produce a whole-colony response. This operates via the comX quorum sensing pathway. Normally, once B. subtilis cells reach a critical population density, their quorum sensing molecules activate global pathways to produce community-wide goods. One such quorum molecule is comX, and at a critical density, it activates pathways which result in the activation of the srfA-promoter (PsrfA). PsrfA activation upregulates the downstream production of surfactant molecules. In our project, we engineered a signal amplifier pathway by placing comX and luxABCDE genes under the control of PsrfA. The result is this: once a critical amount of cells are exposed to the comX molecule, a feed-forward loop is triggered where PsrfA is activated to produce more comX and luxABCDE (BBa_K823025) in a whole-colony response. LuxABCDE was our colorimetric reporter of choice, but it can be replaced with anything you are trying to amplify. For the sake of simplicity, this device just includes PsrfA, comX, and the respective RBS sites to allow for easy integration into whatever your project entails. Just make sure to account for the 4 nucleotides lagging at the end of the 2nd RBS site.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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